Part:BBa_K3285334

mutD5 under pBAD promoter

The gene mutD codes for epsilon subunit of DNA polymerase III. Epsilon subunit is responsible for 3’-5’ proofreading activity of DNA polymerase III. The gene mutD5 is a dominant-negative mutant of mutD, which codes for faulty epsilon subunit. So when expressed, the faulty epsilon subunit gets incorporated into DNA polymerase III which compromises the proofreading activity of DNA polymerase III. Which in turn increases the mutation rate of bacteria.

This composite promoter consists of 2 parts that pBAD-araC promoter and include (Fig.1)

Fig.1: The basic gene circuit of the mutator system

USAGE AND BIOLOGY

In the presence of arabinose, a mutD5 will get expressed increasing the mutation rate of bacteria