Part:BBa_K3183001

Surface (S)-Layer Protein Constitutive Promoter for Lactobacillus sp.

P-slp is a constitutive promoter which can be used in Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species.

The (S)-layer protein (slp) promoter is derived from Lactobacillus acidophilus and is responsible for the high level of transcription of stable mRNAs coding the S-protein monomers^1,2. It was previously characterised in six strains of Lactobacillus reuteri and Lactococcus lactis spp. cremoris MG1363³.
The promoter shows high activity in L. lactis spp. cremoris MG1363, but low activity in L. reuteri.


Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Use by Team Oxford 2019

This part was used in our detection system to express AgrA2 and AgrC2 in L. reuteri. The composite part is: BBa_K3183102.

References

1. Boot, H J et al. “The Lactobacillus acidophilus S-layer protein gene expression site comprises two consensus promoter sequences, one of which directs transcription of stable mRNA.” Journal of bacteriology vol. 178,18 (1996): 5388-94. doi:10.1128/jb.178.18.5388-5394.1996
2. Boot, Hein J., and Peter H. Pouwels. “Expression, Secretion and Antigenic Variation of Bacterial S-Layer Proteins.” Molecular Microbiology, vol. 21, no. 6, 1996, pp. 1117–1123., doi:10.1046/j.1365-2958.1996.711442.x.
3. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.