Coding

Part:BBa_K2972007:Design

Designed by: Huanzhou Yang   Group: iGEM19_BIT-China   (2019-10-16)
Revision as of 20:01, 21 October 2019 by UOH4 (Talk | contribs)


mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mCherry is a red fluorescent protein monomer, which needs to be added with a flexible GS sequence to ensure that its protein expression is not affected.
mCherry's sequence is optimized and can be expressed directly in the E.coli.
When designing mCherry sequence primers, the 20-40 bp region of the primer overlap is recommended.


Source

MCherry red fluorescent protein was isolated from coral (Discosoma) and used as a reporter to characterize protein expression intensity. The sequence of this part was obtained from NCBI, synthesized by GenScript and codon optimized.

References