RNA
sgRNA

Part:BBa_K3286001

Designed by: Paul Alexander Niederau   Group: iGEM19_Wageningen_UR   (2019-10-13)
Revision as of 19:59, 21 October 2019 by AlexNiederau (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

single guide RNA Cas9

Non-targeting Spacer and single guide (sg)RNA scaffold of Streptococcus pyogenes (Spy)Cas9 used and further characterised in the composite Part:BBa_K3286003. The sgRNA is a chimeric non-coding RNA mediating Cas9 activation and target binding and mainly consists of three parts: a 20 nt long spacer binding target DNA, a 42 nt Cas9 binding hairpin and a 40 nt terminator [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None