Device

Part:BBa_K3304000

Designed by: Platon Megagiannis   Group: iGEM19_Thessaloniki   (2019-10-14)
Revision as of 19:54, 21 October 2019 by Karava (Talk | contribs)


Input Gate with NF-κB p65 consensus sequence

This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG (part: ) with initiates the strand displacement cascades. Other auxiliary strands for this gate are BTD Part:BBa_K3304006 and NTB Part:BBa_K3304007 who conclude the reactions and facilitate their trajectory to the desired direction. The output of this gate is TBB (part ) which constitutes the input for the downstream Join Gate (part ). The part comes with two PvuII cut sites and three Nt.BstNBI nicking sites that facilitate the extraction of the gate from plasmid DNA and prepare the double-stranded block for strand displacement reactions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None