Composite
Part:BBa_K3002221
Designed by: Marlene Schlosser Group: iGEM19_TU_Kaiserslautern (2019-10-21)
L2 spectinomycin resistance + cCA_Mut-PETase_SP20His
This composite part contains a spectinomycin resistance (BBa_K3002102) and the mutant PETase with the secretion signal cCA (BBa_K3002120) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2401
Illegal PstI site found at 3485
Illegal PstI site found at 4458 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2401
Illegal NheI site found at 2665
Illegal PstI site found at 3485
Illegal PstI site found at 4458 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2401
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2401
Illegal PstI site found at 3485
Illegal PstI site found at 4458 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2401
Illegal PstI site found at 3485
Illegal PstI site found at 4458
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3220
Illegal NgoMIV site found at 3247
Illegal NgoMIV site found at 4949 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |