Composite
Part:BBa_K3002215
Designed by: Dorothée Klein Group: iGEM19_TU_Kaiserslautern (2019-10-21)
L2 spectinomycin resistance + L1_connector_Pos2 + ccA_MHETase_SP20HA
This composite part contains a spectinomycin resistance (BBa_K3002102) and the MHETase with the cCA (BBa_K3002114) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3738
Illegal PstI site found at 4062
Illegal PstI site found at 4405
Illegal PstI site found at 5215 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2660
Illegal PstI site found at 3738
Illegal PstI site found at 4062
Illegal PstI site found at 4405
Illegal PstI site found at 5215
Illegal NotI site found at 4073 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4983
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3738
Illegal PstI site found at 4062
Illegal PstI site found at 4405
Illegal PstI site found at 5215 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3738
Illegal PstI site found at 4062
Illegal PstI site found at 4405
Illegal PstI site found at 5215
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3671
Illegal NgoMIV site found at 4132
Illegal NgoMIV site found at 4162
Illegal NgoMIV site found at 4477 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |