Composite

Part:BBa_K3002224

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 19:34, 21 October 2019 by Registry (Talk | contribs)

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L2 spectinomycin resistance + cCA_Mut-PETase_SP20-His + ccA_MHETase_HA-SP20HA

L2 spectinomycin resistance + cCA_Mut-PETase_SP20-His + ccA_MHETase_HA-SP20HA This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal cCA (BBa_K3002120) and a SP20His-tag and the wildtype MHETase with the secretion signal cCA (BBa_K3002114) fused with an SP20HA-tag for easy detection via antibody and enhanced secretion.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5348
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6690
    Illegal PstI site found at 7014
    Illegal PstI site found at 7357
    Illegal PstI site found at 8167
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5348
    Illegal NheI site found at 2665
    Illegal NheI site found at 5612
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6690
    Illegal PstI site found at 7014
    Illegal PstI site found at 7357
    Illegal PstI site found at 8167
    Illegal NotI site found at 7025
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5348
    Illegal BglII site found at 7935
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5348
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6690
    Illegal PstI site found at 7014
    Illegal PstI site found at 7357
    Illegal PstI site found at 8167
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5348
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6690
    Illegal PstI site found at 7014
    Illegal PstI site found at 7357
    Illegal PstI site found at 8167
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3220
    Illegal NgoMIV site found at 3247
    Illegal NgoMIV site found at 4949
    Illegal NgoMIV site found at 6623
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None