Composite
YtvA-T
Part:BBa_K143066
Designed by: Chris Hirst Group: iGEM08_Imperial_College (2008-10-26)
YtvA light receptor Protein - Terminator
YtvA light receptor protein (YtvA)(BBa_K143037) coupled to the double terminator (BBa_B0015.
YtvA can be used in conjunction with a sigma factor B specific promoter (BBa_K143010 or BBa_K143011), where the YtvA will act as a receiver for a blue light input to result in a Polymerases per second (PoPS) output.
The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.
The double terminator allows the YtvA to be easily incorporated into a closed transcriptional unit.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 406
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 258
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 157
Illegal SapI site found at 475
[edit]
Categories
Parameters
//chassis/prokaryote/bsubtilis
None |