Composite

Part:BBa_K3002228

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 19:28, 21 October 2019 by MSchlosser (Talk | contribs)


L2 spectinomycin resistance + L1_connector_Pos2 + ccA_MHETase_SP20His

This composite part contains a spectinomycin resistance (BBa_K3002102) and the MHETase with the secretion signal cCA (BBa_K3002123) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2660
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
    Illegal NotI site found at 4073
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4983
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3738
    Illegal PstI site found at 4062
    Illegal PstI site found at 4405
    Illegal PstI site found at 5215
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3671
    Illegal NgoMIV site found at 4132
    Illegal NgoMIV site found at 4162
    Illegal NgoMIV site found at 4477
  • 1000
    COMPATIBLE WITH RFC[1000]


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