Composite
Part:BBa_K3002211
Designed by: Dorothée Klein Group: iGEM19_TU_Kaiserslautern (2019-10-21)
L2 spectinomycin resistance + GLE_Mut-PETase + cCA_MHETase
This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal GLE (BBa_K3002107) and the MHETase with the cCA (BBa_K3002113), both fused with an HA-tag for easy detection via HA-antibody.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5315
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal PstI site found at 6657
Illegal PstI site found at 6981
Illegal PstI site found at 7324
Illegal PstI site found at 8134 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5315
Illegal NheI site found at 2665
Illegal NheI site found at 5579
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal PstI site found at 6657
Illegal PstI site found at 6981
Illegal PstI site found at 7324
Illegal PstI site found at 8134
Illegal NotI site found at 6992 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5315
Illegal BglII site found at 7902 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5315
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal PstI site found at 6657
Illegal PstI site found at 6981
Illegal PstI site found at 7324
Illegal PstI site found at 8134 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2401
Illegal EcoRI site found at 5315
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal PstI site found at 6657
Illegal PstI site found at 6981
Illegal PstI site found at 7324
Illegal PstI site found at 8134
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3238
Illegal NgoMIV site found at 3265
Illegal NgoMIV site found at 4916
Illegal NgoMIV site found at 6590 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |