Composite

Part:BBa_K3002229

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 19:23, 21 October 2019 by Registry (Talk | contribs)

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L2 hygromycin resistance + cCA_WT-PETase_SP20His

This composite part contains a hygromycin resistance (BBa_K3002119) and the WT-PETase with the secretion signal cCA (BBa_K3002124) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2868
    Illegal PstI site found at 3841
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2048
    Illegal PstI site found at 2868
    Illegal PstI site found at 3841
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1408
    Illegal XhoI site found at 250
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2868
    Illegal PstI site found at 3841
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2868
    Illegal PstI site found at 3841
    Illegal NgoMIV site found at 773
    Illegal NgoMIV site found at 955
    Illegal NgoMIV site found at 1326
    Illegal NgoMIV site found at 2603
    Illegal NgoMIV site found at 2630
    Illegal NgoMIV site found at 4332
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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