Composite
Part:BBa_K3002234
Designed by: Marlene Schlosser Group: iGEM19_TU_Kaiserslautern (2019-10-21)
L2 hygromycin resistance 2 + cCA_Mut-PETase_SP20HA
This composite part contains a hygromycin resistance (BBa_K3002127) and the WT-PETase (BBa_K3002109) fused with an SP20HA-tag for easy detectionvia HA-antibody and enhanced secretion.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3453
Illegal PstI site found at 4426 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2633
Illegal PstI site found at 3453
Illegal PstI site found at 4426 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3453
Illegal PstI site found at 4426 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3453
Illegal PstI site found at 4426
Illegal NgoMIV site found at 1270
Illegal NgoMIV site found at 1452
Illegal NgoMIV site found at 3188
Illegal NgoMIV site found at 3215
Illegal NgoMIV site found at 4986 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |