Generator

Part:BBa_K2971002:Design

Designed by: Marthe Ueland   Group: iGEM19_UiOslo_Norway   (2019-10-15)
Revision as of 18:49, 21 October 2019 by Simen (Talk | contribs) (→‎Design Notes)


crtB from Deinococcus radiodurans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 609
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene was selected from Deinococcus radiodurans due to the organism’s extremophilic properties. D.radiodurans exhibit several traits that would make it ideal in our imagined biogenic solar cell (see our design page), such as a high resistance towards ionizing radiation and dehydration. Although the translated protein does not necessarily exhibit the same resistances as the whole organism, it was selected to highlight our interest in D.radiodurans. The gene was placed in an expression vector under pBAD induction so that we could overexpress the protein to test its enzymatic activity. Due to time restraints, and the general difficulty of characterizing carotenogenic enzymes, this was not achieved.

Source

Genomic sequence comes from Deinococcus radiodurans.

References