DNA

Part:BBa_K3046033

Designed by: Philip Srensen   Group: iGEM19_DTU-Denmark   (2019-10-21)
Revision as of 18:47, 21 October 2019 by Lookingforanswer (Talk | contribs)


IS2 downstream sequence for A. niger genome integration

This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the upstream sequence BBa_K3046032).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 118
    Illegal PstI site found at 1262
    Illegal PstI site found at 1863
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 118
    Illegal PstI site found at 1262
    Illegal PstI site found at 1863
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 118
    Illegal BglII site found at 1360
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 118
    Illegal PstI site found at 1262
    Illegal PstI site found at 1863
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 118
    Illegal PstI site found at 1262
    Illegal PstI site found at 1863
    Illegal NgoMIV site found at 1250
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1751
    Illegal SapI site found at 2060


[edit]
Categories
Parameters
None