Composite
CEAker

Part:BBa_K3284001:Design

Designed by: Bella Stevanny, Mutiara Tri Florettira, Ragil Cahyati   Group: iGEM19_SriwijayaGold   (2019-10-20)
Revision as of 18:44, 21 October 2019 by Bellastevanny (Talk | contribs) (→‎Source)


TGFbRI/Taz Chimeric Receptor Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

- In this project, CEAKEER is a synthetically combined receptor used as the diagnostic tool system. TGFBR1, which is originally found in human body, directly interacts with Carcinoembryonic Antigen (CEA). CEA itself functions as an intercellular adhesion molecule and is upregulated in a wide variety of human cancers, including lung cancer. CEA-binding domain mainly lodged in TGFBR1’s extracellular environment. Hence, the domain, boarden between 1th-126th amino acid, was chosen from TGFBR1. Furthermore, Transcriptional Coactivator with PDZ-binding motif (TAZ) consists of Tar receptor, Tar HAMP domain, and EnvZ histidine kinase domain. It presents as an oncogene that is used for the early detection of lung cancer. Therefore, our team selected Taz domains involving 1st-40th and 167th-484th amino acid as part of CEAKEER

Source

The extracellular (CEA-sensing) domain of TGFbRI is a native in Homo sapiens, while the ransmembrane+intracellular domain of Tar/EnvZ receptor is a part from BBa_C0082 Escherichia coli.

References