Part:BBa_J10054:Experience

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Applications of BBa_J10054

Lambert_GA 2019 used this part in experiments to characterize the effect of different strengths of promoters and ribosomal binding sites on expression. The data obtained can aid in the design of constructs by giving information about controlling expression.

Lambert_GA 2019 Characterization

Characterization of medium promoter (BBa_J23106) and strong RBS (BBa_B0034)

Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10054 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10053 and BBa_J10055, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths.

Figure 1: The highlighted points in yellow represent BBa_J10054 (medium promoter/ strong RBS) compared to BBa_J10053 (medium promoter/ weak RBS) and BBa_J10055 (medium promoter/ medium RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter.

Figure 2: The highlighted points represent the BBa_J10054 composite part (medium promoter/ strong RBS).

As the promoter strength increases, the expression increases as well.

The graphs above show that RBS strength has a negligible effect on expression, whereas increased promoter strength increases expression.

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