Composite

Part:BBa_K3110020:Design

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 18:30, 21 October 2019 by Suryazzz (Talk | contribs) (→‎Source)

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Weak Promoter Medium RBS lldR+lldD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1989
    Illegal AgeI site found at 621
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1426


Design Notes

Source

Weak promoter -Medium RBS-lldR flank and lldD flank-terminator were synthesized by IDT
lldR+lldD was amplified from the genome

References

Reference

iGEM ETH zurich 2015
iGEM NUS Singapore 2016