Part:BBa_K3081051

Quorum Sensing CRISPRri System Enables Spatial-level Growth Control and Ultrasensitive Autoregulation of Growth

Based on the well-characterized growth control system, we constructed a quorum sensing CRISPRri system (qs-CRISPRri) to realize smart regulation of bacterial overall states. At first, we use GFP as the output to test our system.

We used different concentration of IPTG to regulate the expression level of LuxI. Grown cultures were induced by different concentration of IPTG in M9 medium for 6hours. And we used flow cytometry to measure the single bacterium’s GFP expression level (Figure.1). The cells’ expression level is positively related to the concentration of IPTG.

Figure 1.Characterization of the quorum sensing system