DNA

Part:BBa_K2940007

Designed by: Ping CHANG   Group: iGEM19_Edinburgh_OG   (2019-10-16)
Revision as of 18:17, 21 October 2019 by Tina0909 (Talk | contribs)


Secreted dye-degrading peroxidase（DyP)


Usage and Biology

Dye-degrading peroxidase (DyP) is from Bacillus subtilis. This part is designed as the control group to check whether the original DyP can be expressed and degrade dyes. So the coding sequence was referenced from NCBI (NC_000964.3) without any modification. (NC_000964.3) It was cloned into pSB1C3 using classical cloning and was verified using SDS-PAGE analysis and ABTS assay.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 10
    Illegal NheI site found at 33
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 155
    Illegal BsaI site found at 164
    Illegal BsaI site found at 1130
    Illegal SapI site found at 560
    Illegal SapI site found at 918
    Illegal SapI.rc site found at 1074




[edit]
Categories
Parameters
None