Regulatory

Part:BBa_K165001:Design

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-26)
Revision as of 09:09, 26 October 2008 by Jszymans (Talk | contribs) (→‎References)

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pGAL1+ w/XhoI sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 469
    Illegal XhoI site found at 477
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 431
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

From the Saccharomyces cerevisiae genome, via Caroline Ajo-Franklin.

References

The activity of this promoter is characterized here:

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=310147


The Gal2 knockout and resulting tunable response in pGAL1 is discussed here:

http://www.jbc.org/cgi/content/abstract/281/19/13485