DNA

Part:BBa_K2940007

Designed by: Ping CHANG   Group: iGEM19_Edinburgh_OG   (2019-10-16)
Revision as of 18:08, 21 October 2019 by Tina0909 (Talk | contribs)


Secreted dye-degrading peroxidase（DyP)

This part is designed as the control group to check whether the original DyP can be expressed and degrade dyes. So the coding sequence was refrenenced from NCBI () without any modification. The strong constitutive promoter and RBS combination were introduced to make the DNA construct. It was cloned into pSB1C3 using classcial cloning and was verified using ABTS assay.

Dye-degrading peroxidase is from Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 10
    Illegal NheI site found at 33
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 155
    Illegal BsaI site found at 164
    Illegal BsaI site found at 1130
    Illegal SapI site found at 560
    Illegal SapI site found at 918
    Illegal SapI.rc site found at 1074


[edit]
Categories
Parameters
None