Part:BBa_K2983062

FadX of Trichosanthes kirilowii

This part is an Yarrowia lipolytica codon optimized version of the bifunctional fatty acid conjugase / desaturase (FadX) from Trichosanthes kirilowii (Uniprot Q84UC0).

Usage and Biology

The bifunctional fatty acid conjugase / desaturase FadX (EC: 1.14.19.16) catalyses the conversion of linoleic acid, C18:2 (9Z,12Z) into punicic acid, C18:3 (9Z, 11E, 13Z) (Figure 1).

Figure 1. Conversion of linoleic acid to punicic acid (both incorporated into phosphatidylcholine) by EC: 1.14.19.16.

The Trichosanthes kirilowii (chinese cucumber / chinese snake gourd) FadX (Tk-FadX) [1] is a 44.918 kDa protein of 387 amino acids. It's activity leads to the accumulation of important quantities of punicic acid in the seeds.

References

[1] Iwabuchi M, Kohno-Murase J, Imamura J. Delta 12-oleate desaturase-related enzymes associated with formation of conjugated trans-delta 11, cis-delta 13 double bonds. J Biol Chem (2003) 278, 4603-4610.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]