Coding

Part:BBa_K2940005

Designed by: Longjiao Chen   Group: iGEM19_Edinburgh_OG   (2019-10-16)
Revision as of 17:59, 21 October 2019 by Tina0909 (Talk | contribs)


Coding Sequence of AzoR with an N-terminal SpyCatcher

Coding sequence for Azoreductase AzoR with an N-terminal SpyCatcher.

Usage and Biology

This part is used for conjugation of AzoR on curli for enzyme immobilisation. The SpyCatcher is designed to be at the N-terminus of the AzoR enzyme. A 2x GGGGS flexible linker was introduced between AzoR and SpyCatcher to make sure the domains of two proteins were not interfering with each other. SpyCatcher sequence were acquired from iGEM registry (part: BBa_K1650037). And the flavin-free, oxygen insensitive azoreductase AzoR was acquired from NCBI (accession number: WP_014229193.1).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 222
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None