Coding

Part:BBa_K3174008:Design

Designed by: Kelsey Hu   Group: iGEM19_Austin_UTexas   (2019-10-09)
Revision as of 17:59, 21 October 2019 by Amacaskill (Talk | contribs)


SYFP2 coding sequence with IS hotspot removed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Conception and Design

The 2015 UT iGEM team originally identified that the SYFP2 gene was especially prone to IS10 element mutations (See their work here).

Design Notes

the redesigned sequence must be less prone to insertion element mutations while still mainting the same amino acid sequence.


Source

The original SYFP2 coding sequence (BBa_K864100)

References