Part:BBa_K3139014:Experience

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Applications of BBa_K3139014

Background

Composite part BBa_K3139014, J23100-fusion protein-B0015-J23106-TEVp-TEVp site-HasA-B0010, anti-Plasmodium system. We choose this part as the best composite part, because its function was the most superior among all the parts of the same type (Figure.1 Lane 3). It also provided significant data for our mathematic model which simulated different promoter combinations (shown in Figure.2) of anti-Plasmodium system and predicted the possibly most superior combination. the amount of both TEV protease and anti-Plasmodium fusion protein should be kept at a certain proportion to avoid a waste of cell resources. If the TEV protease is excessive, it shall be wasted though the fusion proteins are cleaved completely. If the fusion protein is excessive, it cannot be cleaved completely with insufficient TEV protease.

Figure.1 Recombinant plasmid used Figure.2 Mechanism of anti-Plasmodium system

1.Anti-Plasmodium fusion protein.

2.TEV protease with hasA signal peptide, unfolded and nonfunctioning.

3.1 and 2are secreted by has secretion complex.

4.Anti-Plasmodium fusion protein is cleaved by TEV protease and turns functioning.

5.HasA signal peptide is cutted off, the TEV protease is completely functioning. The protease is mostly folded and functioning when it is secreted out of the cell.

User Reviews

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