DNA

Part:BBa_K3280001

Designed by: Amanda Araujo Silva ; Pedro de Carvalho Braga Ilidio Silva ; Lucas Grizotto Ferreira ; Nayla Naomi Kusimoto Takeuti; Joo Paulo Cassucci dos Santos, Paulina Rossi Ferreira ; Giancarlo de Souza   Group: iGEM19_USP_SaoCarlos-Brazil   (2019-10-15)
Revision as of 17:58, 21 October 2019 by Furuta (Talk | contribs)


WspR Diguanylate Cyclase

WspR. This part is a protein domain and increase levels of c-di-GMP to induce biofilm formation.

Characterization

By <a href="">Team iGEM19_USP_SaoCarlos-Brazil 2019</a>

Cyclic di-GMP (c-di-GMP) is recognized as an intracellular signaling molecule coordinating the “lifestyle transition” from motility to sessility and vice versa (i.e. dispersion). The correlation between high c-di-GMP concentration in the cell and biofilm formation or between low c-di-GMP levels and motility has been demonstrated in several bacteria species [1].

Our project include one protein (Iara-alpha) that captures mercury and also produces biofilm to improve the fixation of the heavy metal. So one of our experiments was to characterize several diguanylate cyclases in order to choose the best one for our circuit.

File:Design.svg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 393
    Illegal PstI site found at 465
    Illegal PstI site found at 683
    Illegal PstI site found at 807
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 393
    Illegal PstI site found at 465
    Illegal PstI site found at 683
    Illegal PstI site found at 807
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 361
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 393
    Illegal PstI site found at 465
    Illegal PstI site found at 683
    Illegal PstI site found at 807
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 393
    Illegal PstI site found at 465
    Illegal PstI site found at 683
    Illegal PstI site found at 807
    Illegal NgoMIV site found at 306
    Illegal NgoMIV site found at 636
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 450
    Illegal SapI.rc site found at 273
    Illegal SapI.rc site found at 287


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Parameters
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