Composite

Part:BBa_K2956000

Designed by: Kelly Huang, Michelle Lam, Malini Basu, Neelam Pandya, David Sung, Steven Ionov, Kaavya Ashok, Ali Hasan   Group: iGEM19_NYU_New_York   (2019-10-16)
Revision as of 17:41, 21 October 2019 by Kellyh16 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


RBS-FNS under J23100 Promoter

This is a composite part consisting of a strong promoter, ribosome binding site, and the gene for flavone synthase (FNS). When inserted into a vector and transformed in E. coli, this part allows for the constitutive expression of FNS. FNS converts naringenin to apigenin via a direct 2,3-desaturation of flavanones.

Figure 1: Conversion of Naringenin to Apigenin in FNS B, measured by gas chromatography






































Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 794
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None