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Part:BBa_K2983006

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-11)
Revision as of 17:27, 21 October 2019 by Anthor91 (Talk | contribs) (→‎Usage and Biology)

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SfiI cloning sites

This part is a cloning adapter containing 2 SfiI restriction sites.

Usage and Biology

The Loop Type IIS cloning sites (triangles in Figure 1, see below for detailed information) and two SfiI sites in between to allow, if needed, the insertion of E. coli cloning selection markers like LacZalpha (BBa_K2448003) or reporter RFP (BBa_J04450) expression cassettes.

Figure 1. General architecture of the Yarrowia lipolytica Type IIS RFC[10]-compatible Loop assembly platform.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 12
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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