Part:BBa_K3137005
gntR
The Gluconate repressor GntR, is a transcription factor that negatively regulates the operon involved in the catabolism of d-gluconate via the Entner-Doudoroff pathway and represses genes involved in the different systems related to d-gluconate uptake: gluconate Ⅰ and gluconate Ⅱ.
Usage and Biology
To get more efficient phage resistant parts, we used the ARTP(Atmospheric and room temperature plasma) mutagenesis system and Comparative Genomics to obtain 4 mutant bacterial and 4 key mutant sites (nuoE, yhjH, rzpD And gntR).Resistance tests on these key sites were performed respectively(Fig 1&2).
Since it is not possible to directly see from the figure which component has the least influence on the growth of the bacteria, we use the Grey Relation Analysis (GRA) method to analyze the growth curve (Fig 3) of the bacteria connecting the various components.
We used the Entropy Weight Method (EWM) to determine the weight of each growth point to select the most similarly modified strain (the highest correlation), which is the component that has the least impact on bacterial growth. At the same time, after consulting the industry experts, we revised the weights according to the experts' recommendations to evaluate the components again, making them more in line with the real situation of production, that is, the most suitable components for industrial production. Using GRA's two evaluations of the four components at different weights, we selected the component gntR.(Fig 4)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 425
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |