Composite

Part:BBa_K3110034:Design

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 17:17, 21 October 2019 by Meghamariajacob (Talk | contribs)

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Medium Promoter Strong RBS lldP+lldR+lldD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3650
    Illegal AgeI site found at 464
    Illegal AgeI site found at 927
    Illegal AgeI site found at 1365
    Illegal AgeI site found at 1524
    Illegal AgeI site found at 1536
    Illegal AgeI site found at 2282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3087


Source

Medium promoter - Strong RBS-lldP flank and lldD flank-terminator were synthesized by IDT.
lldP+lldR+lldD were amplified from DH5alpha genome.

References

iGEM ETH zurich 2015
iGEM NUS Singapore 2016
Aguilera, L., Campos, E., Gimenez, R., Badia, J., Aguilar, J., & Baldoma, L. (2008). Dual Role of LldR in Regulation of the lldPRD Operon, Involved in L-Lactate Metabolism in Escherichia coli. Journal of Bacteriology, 190(8), 2997–3005. doi:10.1128/jb.02013-07