Coding

Part:BBa_K3285700

Designed by: Abhinav Masih   Group: iGEM19_IISER-Pune-India   (2019-10-21)
Revision as of 17:12, 21 October 2019 by Abhi masih (Talk | contribs)


Lead Sequestration Protein with RBS

pbrD is a protein that sequesters lead. It is present downstream of a strong RBS (BBa_B0034) and binds to lead ions in the cytoplasm and decrease its concentration.

Usage and Biology

This is an intermediate part which can be incorporated under a promoter. The pbrD protein is known to sequester lead ions in the cytoplasm of the bacterial cell.

When put under a lead responsive promoter, the part is expected to show dosage dependent sequestration properties.

Construction

File:PbrD Gel.jpeg

The part was artificially synthesised with corresponding primers that have overlap with GFP sequence. This allows it to be put downstream of the lead dependent biosensor <a href="https://parts.igem.org/Part:BBa_K1758333">(BBa_K1758333)</a>. This part has been synthesised with a strong Ribosome Binding Site <a href="https://parts.igem.org/Part:BBa_B0034">(BBa_B00340)</a> upstream of the coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 532
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None