Part:BBa_K592002

ccaR

ccaR is the response regulator of the green sensor (BBa_K592001) that also includes the membrane-associated histidine kinase ccaS. The two-component system is also dependent of a chromophore.

<img src=“https://2019.igem.org/wiki/images/9/96/T--NYU_New_York--results1.png“>

Figure 1: Plates containing the pSR58.6 (left) and pNO286-3 (right) plasmids under UV light. The pSR58.6 plasmid contains CcaR and superfolder GFP. The pNO286-3 plasmid contains the mini-CcaS gene, along with ho1 and pcyA.

<img src=“https://2019.igem.org/wiki/images/a/a3/T--NYU_New_York--results2.jpg”>

Figure 2: Plate containing cells co-transformed with both pSR58.6 and pNO286-3 plasmids.

<img src=“https://2019.igem.org/wiki/images/6/60/T--NYU_New_York--results3.png”>

Figure 3: Light sensor readings from plates containing optogenetic plasmids

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 402
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]