Part:BBa_K2940001

PelB and Bpul

The PelB leader sequence was joined to Bpul laccase to enable exportation of the enzyme into the periplasmic space. Both parts were isolated from the iGEM kit via Q5 PCR and joined together using Gibson Assembly. Not only was exporting Bpul outside of the cell vital for our overall project, it also allowed us to assess the activity of Bpul without the need for expensive and time consuming purification steps.

Characterisation