Composite

Part:BBa_K3081052

Designed by: Yiqiang Shi   Group: iGEM19_Peking   (2019-10-21)
Revision as of 16:55, 21 October 2019 by Registry (Talk | contribs)

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A receiver device of AHL (change terminator)

Quorum sensing system is widely used in gene circuit designed as a sensor of cell density. As a transcription factor, LuxR senses AHL and then activates the transcription of plux. In our project, we want dCas9 to express when bacteria density is high. And we put the GFP gene under the control of plux, and get this composite part. We used this part to test the quorum sensing system. To avoid the homologous recombination, we changed the terminator of GFP to B1002.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 1705


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