Part:BBa_J10055:Experience

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Applications of BBa_J10055

Lambert 2019 used this part in experiments to model the effect of different promoter strengths and ribosomal binding strengths to develop a model to aid in controlling expression in constructs.

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Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10055 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10053 and BBa_J10054, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths.

Figure 1: The highlighted points in yellow represent BBa_J10055 (medium promoter/ strong RBS) compared to BBa_J10054(medium promoter/ medium RBS) and BBa_J10053(medium promoter/ weak RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter.

Figure 2: The highlighted points represent the BBa_J10055 composite part (medium promoter/ strong RBS). As the promoter strength increases, the expression increases as well.</center>

The graphs above show that RBS strength has a negligible effect on expression, whereas increased promoter strength increases expression. UNIQ9ca08a0a564b30e1-partinfo-00000000-QINU

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