Composite

Part:BBa_K2949012

Designed by: Yujie Wang   Group: iGEM19_AHUT_China   (2019-08-12)
Revision as of 16:35, 21 October 2019 by Wany (Talk | contribs)


Carbonic Anhydrase 2(L203K)-N-LCTASR

we constructed this new biobrick by connecting the N-terminal of the mutant human carbonic anhydrase 2 [CA2(L203K)] (BBa_K2547004) with the six-residue sulfatase submotif (LCTPSR). Formylglycine generating enzyme can selectively recognize and oxidize the cysteine residues in the sulfatase subunit of the protein terminal to form formylglycine (FGly) residues containing aldehyde groups, which can be immobilized via forming covalent bond with amino functionalized carriers (Unisil 30-100 NH2) through the Schiff base reaction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None