Part:BBa_K274120:Experience

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Characterization by UiOslo_Norway 2019

This part was of special interest to our team. The part consists of homologs of the genes we used; crtE, crtB and crtI from Deinococcus radiodurans. BBa_K274120 has individual ribosomal binding sites for each individual enzyme. The part that we made had a single ribosomal binding site followed by all three genes in a single open reading frame. The first two genes had their stopcodons removed (see BBa_K2971004 and BBa_K2971010). Figure 1 shows the result of expressing BBa_K274120 in E.coli (BL21) for 24 hours. The red coloration in the induced pellet is caused by the presence of lycopene. The induced culture with BBa_K274120 produced significant amount of lycopene when compared to expression of BBa_K2971004 (figure 2).

Figure 1: Induction of BBa_K274120 in Escherichia coli (BL21)
Expression was induced in 2% arabinose
From left to right :Uninduced E.coli with empty expression vector, Induced E.coli with empty expression vector, uinduced E.coli with BBa_K274120, induced E.coli with BBa_K274120.

Figure 2: Expression of crtEBI in Escherichia coli (BL21)
Expression was induced in 2% arabinose
From left to right :Uninduced E.coli with empty expression vector, Induced E.coli with empty expression vector, induced E.coli with crtEBI expression vector, uninduced E.coli with crtEBI expression vector.

UNIQ7f3bd3b38cb04511-partinfo-00000001-QINU UNIQ7f3bd3b38cb04511-partinfo-00000002-QINU