Composite

Part:BBa_K3002100:Design

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 16:28, 21 October 2019 by Kleind (Talk | contribs)

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Level 1 - Mutant PETase + cCA + SP20 HA (Phytobrick)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1909
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002027 (PAR-promoter), BBa_K3002006 (RPL23-Terminator), BBa_K1921003 (mutant PETase (Mutate M) of the iGEM team TJUSLS China), BBa_K3002007 (cCA secretion signal), BBa_K3002010 (SP20HA-tag), BBa_K3002302 (connector B1-B2), BBa_K3002303 (connector B2-B3), BBa_K3002304 (connector B4-B5) and BBa_K3002305 (connector B5-B6)

References