Coding

Part:BBa_K2972005:Design

Designed by: Huanzhou Yang   Group: iGEM19_BIT-China   (2019-10-16)
Revision as of 16:06, 21 October 2019 by UOH4 (Talk | contribs) (→‎Source)

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GGPP synthase (crtE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 223
    Illegal AgeI site found at 96
    Illegal AgeI site found at 718
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The crtE protein is mainly composed of a helix and an irregular coil. Its hydrophobic cavity is a key site for binding and catalysis to the substrate, and mutation of its key site is expected to increase the activity of the enzyme.

Source

The sequence of this part is obtained from NCBI and it comes from an E.coli's genome which intergrated lycopene gene.

References