Composite

Part:BBa_K3002100

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 16:05, 21 October 2019 by Registry (Talk | contribs)

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Level 1 - Mutant PETase + cCA + SP20 HA (Phytobrick)

This composite part contains the PAR-promoter of the team iGEM17_NU_Kazakhstan (BBa_K2516001) in combination with the RPL23-Terminator (BBa_K3002006), a HA-tag (BBa_K3002017) and the coding sequence of the mutant PETase (BBa_K3002014) plus the MoClo connectors for positions B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1909
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None