Composite
Part:BBa_K3002100
Designed by: Dorothée Klein Group: iGEM19_TU_Kaiserslautern (2019-10-21)
Level 1 - Mutant PETase + cCA + SP20 HA (Phytobrick)
This composite part contains the PAR-promoter of the team iGEM17_NU_Kazakhstan (BBa_K2516001) in combination with the RPL23-Terminator (BBa_K3002006), a HA-tag (BBa_K3002017) and the coding sequence of the mutant PETase (BBa_K3002014) plus the MoClo connectors for positions B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 249
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1909
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |