Composite

Part:BBa_K3110047:Design

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-15)
Revision as of 16:03, 21 October 2019 by Meghamariajacob (Talk | contribs) (→‎Source)


Promoter RBS FimH 136KO-RPMrel


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 549
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

FimH 136KO-RPMrel was optained from Biobrick. Promoter+RBS and terminator were obtained as gBlock from IDT. These were joined together using SOEing (Spliced Overlap Extenstion) PCR.

References