DNA

Part:BBa_K2940001

Designed by: Nathan Wu   Group: iGEM19_Edinburgh_OG   (2019-10-15)
Revision as of 16:01, 21 October 2019 by Nw nathan (Talk | contribs)


PelB and Bpul

The PelB leader sequence was joined to Bpul laccase to enable exportation of the enzyme into the periplasmic space.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 195
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 1030
    Illegal AgeI site found at 318
    Illegal AgeI site found at 1195
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None