Composite

Part:BBa_K3110041:Design

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 15:57, 21 October 2019 by Meghamariajacob (Talk | contribs) (→‎References)

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lldRO1-J23117-lldRO2 Medium RBS sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 175


Design Notes

None.


Source

sfGFP along with terminator was obtained from the Biobrick. lldRO1-J23117-lldRO2 amd B0032 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extention) PCR.

References

iGEM ETH zurich 2015
iGEM NUS Singapore 2016