Part:BBa_K2908676

s(GATA3)p-GAD-miR101-BS

The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of GAD and miR101-BS for sequence-specific expression of GAD.Therefore,we used our p(GATA3)p(BBa_K2908000) as a specific promoter. At the same time we used our miR101-BD(BBa_K2908668) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation domain of GAD.In this way we can ensure the specificity and independence of this protein. New part: s(GATA3)p-GAD-miR101-BD (BBa_K2908676) [GAD is totally the same as Gal4-VP16]
The part improved in the specificity in Triple negative cancer (TNBC). Existing part: CMV-Gal4-VP16 (BBa_K2580666)
The part has low specificity in the TNBC cells compared to normal cells although the team members who created the part stated that it is a cancer-specific part.
As for this new part we created this year, it contains the brand new synthetic promoter we designed (BBa_K2908000), the existing sequence in the previous iGEM parts, GAD (Gal4-VP16), and the new designed part miR101-BD. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 99
  Illegal NheI site found at 584
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 360
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 279