Composite

Part:BBa_K2956000:Design

Designed by: Kelly Huang, Michelle Lam, Malini Basu, Neelam Pandya, David Sung, Steven Ionov, Kaavya Ashok, Ali Hasan   Group: iGEM19_NYU_New_York   (2019-10-16)
Revision as of 15:53, 21 October 2019 by Kellyh16 (Talk | contribs) (→‎Design Notes)


RBS-FNS under J23100 Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 794
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a composite part consisting of BBa_J23100 promoter, BBa_B0034 ribosome binding sequence, and BBa_K2956000 coding region for flavone synthase (FNS). These parts were assembled together to allow for constitutive expression of the FNS gene which converts naringenin to apigenin. When inserted into a vector plasmid and expressed in E. coli, naringenin was shown to be converted at a rate that was positively correlated to cell growth, demonstrating the efficacy of this system.

Source

This part contains flavone synthase (FNS) from Gerbera hybrida.

References