Coding

Part:BBa_K2924022:Design

Designed by: Arian Abbasi   Group: iGEM19_Duesseldorf   (2019-10-13)
Revision as of 15:49, 21 October 2019 by AndreasN (Talk | contribs)

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accA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 517
    Illegal SapI.rc site found at 714


Design Notes

The gene from E. Coli K12 was cloned into pet21a in an operon-like manner to ensure transcription of all genes of the complex at the same level and from the same vector.



Source

The gene was cloned from the genome of E. coli K12

References