Measurement

Part:BBa_J10051:Experience

Designed by: Malcolm Campbell   Group: iGEM2005   (2012-01-04)
Revision as of 15:28, 21 October 2019 by Janetstandeven (Talk | contribs) (→‎Applications of BBa_J10051)


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Applications of BBa_J10051

Lambert 2019 used this part in experiments to model the effect of different promoter strengths and ribosomal binding strengths to develop a model to aid in controlling expression in constructs.

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Lambert_GA 2019 Characterization

Characterization of weak promoter (BBa_J23113) and strong RBS (BBa_B0034)

T--Lambert GA--tuning3b.png


Figure 1. The highlighted points in yellow represent BBa_J10051 (weak promoter/ strong RBS) compared to BBa_J10050(weak promoter/ weak RBS) and BBa_J10052(weak promoter/ medium RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter.



T--Lambert GA--tuning3a.png


Figure 2. The highlighted points represent the BBa_J10051 composite part (weak promoter/ strong RBS). As the promoter strength increases, the expression increases as well.


The graphs above show that RBS strength has a negligible effect on expression, whereas increased promoter strength increases expression.




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