Composite

Part:BBa_K3259002

Designed by: Tsuruta Soh   Group: iGEM19_TokyoTech   (2019-10-14)
Revision as of 15:25, 21 October 2019 by Crane (Talk | contribs)


Contructs for characterization of pYcgZ-Rhl hybrid promoter

This construct was designed to quantify BBa_K3259000. BBa_K3259000 is expected to be induced when both blue light and RhlR /PAI1 complex are present.

This construct was created by combining parts with GFP downstream of BBa_K3259000 and parts that constitutively produce RhlR.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 899
    Illegal NheI site found at 922
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1193
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1668
    Illegal BsaI.rc site found at 808


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Categories
Parameters
None