Coding

Part:BBa_K2996502

Designed by: Shifeng Jiang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-03)
Revision as of 15:04, 21 October 2019 by Jsf19981231 (Talk | contribs)


C-luciferase is put downstream J23119

We used the C-terminal part of firefly luciferase in order that we could fuse it with part BBa_K2996500.The fused protein could bind specific DNA sequence to bring the C-luciferase near the other part of N-terminal part of firefly luciferase.This was aimed to signal on/off target of dCas9.In order to make the trascription of this part steady,we chose J23119 as the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 302
    Illegal AgeI site found at 441
  • 1000
    COMPATIBLE WITH RFC[1000]


Figure 1. PCR product of C-luciferase with J23119 promoter upstream(542bp). The ladder represents the size of DNA in bp.
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Parameters
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